In an ongoing effort to develop new assays that replace radioactive methods, several different approaches have been advanced. Our objective was to automate a commercially available chemiluminescent assay for the measurement of cellular cyclic adenosine monophosphate levels. A script (Gemini 3.1) written for a Genesis RSP 8/150 with ROMA (TECAN AG) pipetting station directed the fully automated chemiluminescent assay (cAMP-Screen, Cyclic AMP ELISA System, TROPIX). A specially designed reagent block was used to hold the various reagents contained in the kit. Liquids were handled using fixed tips coated with teflon while movement of the microtiter plates was done by the ROMA. Peripherals included an EMBLA 96/384 well washer and a Tecan Ultra plate reader.
Conclusion: We developed a fully automated high throughput chemiluminscent assay that measures cellular cyclic adenosine monophosphate levels.